Explanation:

- Option 1: Basic

- Developed in the 1960s, primarily for beginners in programming.

- Not used in second generation computers.

- Option 2: Pascal

- Designed in the late 1960s for teaching structured programming.

- Not associated with second generation computers.

- Option 3: Machine Language

- The lowest-level programming language.

- Used in first-generation computers.

- Option 4: Algol

- Developed in the late 1950s, used for scientific computations.

- Relevant but not primarily used by second generation computers.

- Option 5: Assembly Language

- A low-level programming language.

- Used extensively in second generation computers for programming.