Explanation:

- Option 1: Ghiyasuddin Balban: He was a Sultan of Delhi from the Mamluk dynasty and not related to the Mughals.

- Option 2: Genghis Khan: The Mughals were descendants of both Genghis Khan through their maternal line and Timur (Tamerlane) through their paternal line. Babur, the founder of the Mughal Empire, was a direct descendant of Timur and claimed descent from Genghis Khan.

- Option 3: Mir Jumla: He was a general and not an ancestor of the Mughals. He served under the Mughal emperor Aurangzeb.

- Option 4: Muhammad Tughluq: He was a Sultan from the Tughlaq dynasty, unrelated to the Mughal ancestry.