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Consider the following techniques/phenomena :
1. Budding and grafting in fruit plants
2. Cytoplasmic male sterility
3. Gene silencing
Which of the above is/are used to create transgenic crops ?
1 only
2 and 3 only
1 and 3 only
None
Transgenic plants are the ones, whose DNA is modified using genetic engineering techniques. The aim is to introduce a new trait to the plant which does not occur naturally in the species.
Grafting and budding are horticultural techniques used to join parts from two or more plants so that they appear to grow as a single plant. In grafting, the upper part of one plant grows on the root system (rootstock) of another plant. In the buddingprocess, a bud is taken from one plant and grown on another. It do not lead to any genetic modification.
Cytoplasmic male sterility is total or partial male sterility in plants as the result of specific. Cytoplasmic–genetic male sterility systems are widely exploited in crop plants for hybrid breeding due to the convenience of controlling sterility.
Gene silencing using RNA interference technology, transcriptional gene silencing, and virus-induced gene silencing has been used in horticultural crops. The presence of double-strand RNAs inside the cell lead to the production of small interfering RNAs, short hairpin RNAs, and microRNAs.
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