Consider the following statements regarding lsquo Luciferase rsquo 1 It is an enzyme that gives fire...........

Science Affairs Biotechnology

Download Solution PDF

Consider the following statements regarding ‘Luciferase’

1. It is an enzyme that gives fireflies their glow

2. It can be used for low light intensity indoor lighting

3. It can be used to transform trees into self-powered street lights

Which of the above statements are correct?

1 only

1&2 only

2&3 only

All of the above

छोटे Courses बड़े Results

Master Biotechnology

Prepare through Micro-courses

Try Now

Explanation:

Luciferase
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein.
Luciferases are widely used in biotechnology, for microscopy and as reporter genes, for many of the same applications as fluorescent proteins. However, unlike fluorescent
proteins, luciferases do not require an external light source, but do require addition of luciferin, the consumable substrate.
Luciferases can be produced in the lab through genetic engineering for a number of purposes. Luciferase genes can be synthesized and inserted into organisms or transfected into cells. Mice, silkworms, and potatoes are just a few of the organisms that have already been engineered to produce the protein.
In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by light sensitive apparatus such as a luminometer or modified optical microscopes.
Think
How is Luciferase different from Photoprotein?