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Fermentation is a metabolic process that converts sugar to acids, gases, or alcohol. Dark fermentation is the fermentative conversion of organic substrate to biohydrogen. It is a complex process manifested by diverse groups of bacteria. Biohydrogen is defined as hydrogen produced biologically, most commonly by algae, bacteria and archaea. Biohydrogen is a potential biofuel obtainable from both cultivation and from waste organic materials. Dark fermentation differs from photofermentation in that it proceeds without the presence of light. Utilization of wastewater as a potential substrate for biohydrogen production has been drawing considerable interest in recent years especially in the dark fermentation process
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